There is no right or wrong answer to this. If you are currently taking a vitamin D supplement and want to check the impact of your supplementation routine, then you can take the test straight away.
However, if you’re looking to discover your ‘baseline’ (diet and lifestyle alone), we recommend a washout period of 2 weeks (Iron and B12) and 4-6 weeks (Vitamin D).
Always consult your GP or healthcare professional before making a change to your supplementation plan.